FIG. 1.

Transcriptional effects of Romi, 5-Azacytidine (5-AzC), and transcription factor (TF) on islet-specific hormones, TFs, and transporters. Real-time PCR analysis of gene expression in human dermal fibroblasts (hDFs) undergoing infection by adenoviral pancreatic and duodenal homeobox 1 (Pdx1) for 72 h and/or addition of 30 nM Romi and 1 μM 5- 5AzC for 48 h. Data are expressed relative to GAPDH mRNA. Values are the mean±S.E. from three separate experiments, each analyzed in duplicate. *, statistical significance with P<0.05 value using a Student's t-test.