FIG. 3.

Glucagon and insulin are produced and secreted from hDFs undergoing the reprogramming protocol. (a) Left panel—immunolabeling of glucagon (red) and c-peptide (green). Right panel—quantification of cells stained positive for each antibody tested. *, statistical significance with P<0.05 value using a Student's t-test. (b) Enzyme-linked immunosorbent assay of the relative amount of insulin secreted versus the cellular insulin content in control hDFs and hDFs that have undergone the reprogramming protocol. (c) The ratio of glucagon secreted per cellular content of glucagon as measured by radioimmunoassay with different glucose concentrations in control cells and hDFs undergoing the reprogramming protocol. Data represent percent of hormones secreted versus the hormone content. Values are the mean±S.E. from three separate experiments. *, statistical significance with P<0.05 value using a Student's t-test.