. 2013 Sep;10(3):326–337. doi: 10.1089/zeb.2013.0876

Table 2.

Detection Limit and Linear Range of Folates

Folate	Detection limit (pmole)^a	Recovery rate (%)^b	Linear range (pmole)^b
THF/5,10-CH₂-THF	0.2	99.0±9.9	0.2–18.6
5-CH₃-THF	0.2	103±16.3	0.2–12.5
10-CHO-THF	0.12	88±12.5	0.12–10.2
DHF	0.5	100±15.1	0.6–6.0

^{^a}

The detection limit refers to the lowest concentration of folate in the 50 μL sample injected for HPLC analysis.

^{^b}

The recovery rate and linear range were determined when folate standards were added to the supernatant of embryo homogenates before the conversion enzymes were added. The concentrations of the standard folate added are, 2 pmole for THF, 1.5 pmole for 5-CH₃-THF, 1.6 pmole for 10-CHO-THF, and 4.5 pmole for DHF.