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. 2013 Oct;23(5):332–343. doi: 10.1089/nat.2013.0424

FIG. 7.

FIG. 7.

A comparison of gene expression between induced 293FT cells and hiPSCs. (a) Relative levels of transcriptional of each gene in 293FT cells transfected with siRNA for small RNA-197 (si-small RNA-197) were evaluated and compared with expression levels in hiPSCs using 1-step real-time qRT-PCR. Top: 48 hours after transfection. Bottom: cells were maintained in ES medium for 2 weeks after transfection. Although RGM249 was expressed at high levels even 2 weeks after transfection in 293FT cells treated with si-small RNA-197; other genes were expressed at similar levels as in hiPSCs. (b) Relative small RNA-197 expression levels were determined in hiPSC, small-RNA-197-targeted siRNA-induced, and 293FT cells by 2-step real-time qRT-PCR (n=5). The expression levels in transfectants were similar to those in hiPSCs. Effective small RNA-197 silencing was confirmed in transfectants. (c) Translation of hTERT, p53, and Oct4 was examined in hiPSCs and si-small RNA-197-induced 293FT and 293FT 24 hours after transfection. p53 expression was upregulated in treated cells, and Oct4 was expressed at similar levels in all cells, except for 293FT cells. hTERT was not regulated in cells with siRNA or small RNA in a fixed manner.