Table 1.
Effect of the Anti-rpoA and Anti-rpoD PNAs on Growth of Listeria monocytogenes in Pure Culture
| |
5 μM |
10 μM |
15 μM |
30 μM |
40 μM |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| PNA Name | Log CFU (±SD) | Reduction log from H2O | Log CFU (±SD) | Reduction log from H2O | Log CFU (±SD) | Reduction log from H2O | Log CFU (±SD) | Reduction log from H2O | Log CFU (±SD) | Reduction log from H2O |
| Anti-rpoA | 4.96±(0.31) | 1.31* | 4.30±(0.13) | 1.97* | 3.83±(0.23) | 2.44* | 3.52±(0.29) | 2.68* | Cleared | 6.16* |
| Anti-rpoD | 6.09±(0.06) | 0.19 | 5.79±(0.13) | 0.48* | 5.43±(0.09) | 0.85* | 3.69±(0.32) | 2.50* | Cleared | 6.16* |
| Control PNA |
6.63±(0.12) | −0.36 | 6.53±(0.05) | −0.26 | 6.14±(0.25) | 0.14 | 5.23±(0.26) | 0.97* | 4.44±(0.33) | 1.71* |
| Water | 6.27±(0.59) | 0 | 6.27±(0.59) | 0 | 6.27±(0.59) | 0 | 6.19±(0.22) | 0 | 6.16±(0.87) | 0 |
The peptide nucleic acids (PNAs) were incubated with 5×103 L. monocytogenes at 37°C for 8 hours. Colony forming units (CFU) were counted on trypticase soy agar.
*
Values found to be significantly different (p≤0.05) from water by statistical analysis (analysis of variance and Fisher's least significant difference comparisons test).
CFU, colony forming units; SD, standard deviation.