Table 2.
Effect of the Combined Anti-rpoA and Anti-rpoD PNAs on Growth of L. monocytogenes in Pure Culture
| |
5/5μM |
10/10μM |
15/15μM |
|||
|---|---|---|---|---|---|---|
| PNA Name | Log CFU (±SD) | Reduction log from H2O | Log CFU (±SD) | Reduction log from H2O | Log CFU (±SD) | Reduction log from H2O |
| Anti-rpoA/Anti-rpoD | 3.58±(0.20) | 1.81* | Cleared | 5.39* | Cleared | 5.39* |
| Control PNA | 5.38±(0.39) | 0.01 | 5.20±(0.20) | 0.18 | 5.02±(0.09) | 0.36 |
| Water | 5.39±(0.36) | 0 | 5.39±(0.36) | 0 | 5.39±(0.36) | 0 |
The PNAs were incubated with 8×103 L. monocytogenes at 37°C for 8 hours. CFU were counted on TSA.
*
Values found to be significantly different (p≤0.05) from water by statistical analysis (analysis of variance and Fisher's least significant difference comparisons test).