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Proliferation of expanded CB cells. CB-CD34+ were maintained in 4 different cultures media: 2 serum-containing media (4F cIMDM, 5F cIMDM) and 2 serum-free media (4F KSR, 5F KSR). (a) Proliferation assay, (b) growth profile of total CD34+CD38− cells, (c) CD133+CD38− cells, and (d) CD34−CD38+ cells. After culturing the cells for 5 and 7 days, cells were harvested for analysis and normalized with day 0 (n = 3). *P < 0.001, **P < 0.02, and ***P < 0.05.
