. 2013 Aug 20;2013:909812. doi: 10.1155/2013/909812

Table 2.

Folds expansion of CD34⁺CD38⁻, CD133⁺CD38⁻, and CD34⁻CD38⁺ cells under various cytokine culture conditions.

Culture conditions			Day	Fold expansion
Name	Medium	Cytokines	Day	CD34⁺CD38⁻	CD133⁺CD38⁻	CD34⁻CD38⁺
4F cIMDM	IMDM + FBS	Flt3-L, SCF, TPO, and IL-6	5	2.6 ± 1.1	0.7 ± 0.4	12.0 ± 1.4
4F cIMDM	IMDM + FBS	Flt3-L, SCF, TPO, and IL-6	7	4.2 ± 1.3	3.1 ± 1.6	62.8 ± 5.0
5F cIMDM	IMDM + FBS	Flt3-L, SCF, TPO, IL-6, and Wnt1	5	3.9 ± 1.0	2.5 ± 1.2	9.3 ± 1.3
5F cIMDM	IMDM + FBS	Flt3-L, SCF, TPO, IL-6, and Wnt1	7	6.6 ± 1.4	6.0 ± 2.6	89.7 ± 12.2***
4F KSR	IMDM + KSR	Flt3-L, SCF, TPO, and IL-6	5	6.3 ± 1.6	4.7 ± 2.0	7.6 ± 2.2
4F KSR	IMDM + KSR	Flt3-L, SCF, TPO, and IL-6	7	18.5 ± 0.4*	11.5 ± 3.4***	20.1 ± 6.8*
5F KSR	IMDM + KSR	Flt3-L, SCF, TPO, IL-6, and Wnt1	5	5.9 ± 1.2	5.4 ± 2.2	8.4 ± 2.2
5F KSR	IMDM + KSR	Flt3-L, SCF, TPO, IL-6, and Wnt1	7	24.3 ± 2.1^∗,∗∗	12.3 ± 4.0***	13.0 ± 3.7*

Data represented as mean fold increasing in expansion of three independent CB sample ± SD expanded for 5 and 7 days subtracted with day 0 (n = 3). *P ≤ 0.001 versus 4F cIMDM and 5F cIMDM, **P = 0.005 versus 4F KSR, ***P < 0.05 versus 4F cIMDM on the same day of culture.