Figure 2.

Spectral properties of lancelet FPs. (a) Normalized excitation and emission spectra of laGFP and mature laRFP. (b) The change in the laRFP emission spectrum during the course of its maturation. (c) The change in the laRFP absorption spectrum during the course of its maturation from the green (green line) to the red (red line) state. Spectra were measured at 8 h intervals except for the final curve, which was measured on the fifth day. (d, e) The pH-dependence of the laRFP absorption spectrum. (f) Normalized excitation and emission spectra of the mutant protein laGFP-Red.