Table 2. Spectral characteristics.
λex (nm) | λem (nm) | |
---|---|---|
laRFP mutants | ||
WT laRFP | 521 | 592 |
ΔSer83 | 514 | 594 |
Tyr62Ser | 514 | 524 |
Tyr62Gln | 514 | 526 |
Tyr62Phe | 511 | 526 |
Gln35Met/Leu | 504 | 523 |
Cys140Ser | 500 | 518 |
Cys140Asn | 488 | 523 |
Gln211Glu | 512 | 521 |
Pro55His | 494 | 511 |
Pro55Ala | 490 | 520 |
laGFP mutants† | ||
WT laGFP | 502 | 511 |
a | 505 | 514 |
a + b | 505 | 514 |
a + b + c | n/d | n/d |
a + b + c + d (laGFP-Red) | 512/518 | 523/591 |
a, Thr142Asp + Asp154Gly; b, Glu211Gln + Glu35Gln; c, Gln56Asn + Val87Met ; d, Gly101Ser + Tyr103His.