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. 2013 Sep 3;7:139. doi: 10.3389/fncir.2013.00139

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Copyright © Kilb, Kirischuk and Luhmann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional expression of ρ subunit containing GABA_A receptors in the intermediate zone. Microfluorimetric registration of Ca²⁺ transients induced by application of 100 μM GABA and 100 μM of the ρ subunit specific agonist cis-4-aminocrotonic acid (CACA) in neurons from the intermediate zone (A) and cortical plate (B). Note that CACA induced Ca²⁺ transients occurred only in the intermediate zone (A₁,B₁) that the ρ subunit containing GABA_A receptor antagonist (1,2,5,6-tetrahydropyridin-4-yl)met-hylphosphinic acid (TPMPA) was inefficient in the cortical plate (A₂,B₂) and that the GABA_A antagonist bicuculline (BMI) completely abolished GABAergic Ca²⁺ transients in the CP (A₃,B₃). Modified from Denter et al. (2010).