Skip to main content
. Author manuscript; available in PMC: 2013 Dec 20.
Published in final edited form as: Cell. 2013 Jun 20;153(7):1461–1474. doi: 10.1016/j.cell.2013.05.037

Figure 5. Mammalian stress granule clearance is impaired by defective autophagy.

Figure 5

A.) atg7−/− MEFs showed a low level of cells with clearly visible stress granules even in the absence of exogenous stressors. B.) Heat shock for 1hr at 43°C resulted in robust stress granule formation in both WT and atg7−/− MEFs. C.) After shift from 43°C back to 37°C stress granules clear within minutes in wild type MEFs, but persist for the duration of the assay (2hrs) in atg7−/− MEFs. Scale bar equals 10μm. D) Quantitation of the data in A-C, mean values based on a minimum of 3 replicate experiments +/− SEM. E) Real time quantitative PCR verifies the expression levels of atg7 in wild type and atg7−/− MEFs. See also Figure S7.