Table 2.
Name of primers | Sequences of primers (5′-3′) |
---|---|
PstI adapter | Sense strand: CTC GGA AGC CTC AGT CCC AGA CTG CGT ACA TGC A-OH |
Antisense strand: phos-TGT ACG CAG TCT GGG ACT GAG GCT TCC GAG A-OH | |
| |
MseI adapter | Sense strand: GAG CAA GGC TCT CAC AAG GAC GAC CGA CGA G-OH |
Antisense strand: phos-TAC TCG TCG GTC GTC CTT GTG AGA GCC TTG CT-OH. | |
| |
MseI suppressed amplification primer | GAG CAA GGC TCT CAC A |
| |
PstI suppression amplification primer | CTC GGA AGC CTC AGT C |
| |
MseI preamplification primer | GAC GAC CGA CGA GTA AC |
| |
PstI preamplification primer | AGA CTG CGT ACA TGC AGG A |
| |
PstI SAM primers | Adapter 1: AGA CTG CGT ACA TGC AGG ACC |
Adapter 2: AGA CTG CGT ACA TGC AGG ACG | |
Adapter 3: AGA CTG CGT ACA TGC AGG AGC | |
Adapter 4: AGA CTG CGT ACA TGC AGG A CT | |
Adapter 5: AGA CTG CGT ACA TGC AGG A TC | |
Adapter 6: AGA CTG CGT ACA TGC AGG A CA | |
Adapter 7: AGA CTG CGT ACA TGC AGG A AC | |
Adapter 8: AGA CTG CGT ACA TGC AGG A GT | |
Adapter 9: AGA CTG CGT ACA TGC AGG A TG | |
Adapter 10: AGA CTG CGT ACA TGC AGG A GA | |
Adapter 11: AGA CTG CGT ACA TGC AGG A AG | |
Adapter 12: AGA CTG CGT ACA TGC AGG A AT | |
| |
5′-Anchored SSR primers | PAC: a: KKR YRY YAC ACA CAC ACA C |
b: KKY RYR YCA CAC ACA CAC A | |
PCT: a: KKV RVR VCT CTC TCT CTC T | |
b: KKR VRV RTC TCT CTC TCT C |
K = G/T, R = G/A, Y = T/C, V = G/C/A, H = A/C/T for 5′-anchored SSR primer sequences.