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. 2013 Jul 18;(77):50456. doi: 10.3791/50456
Measurement Name Description
Time Image frame number
Area Total number of pixels comprising the region
Volume (4/3)πabc, where a = ½ major axis length of region, b = ½ minor axis length, and c = b or ½ "Chamber height" (whichever is smaller)
(X)_(Y)mean Mean pixel intensity for the region mask (Y) in color channel (X)
(X)_(Y)median Median pixel intensity for the region mask (Y) in color channel (X)
(X)_(Y)std Standard deviation of pixel intensities for the region mask (Y) in color channel (X)
(X)_(Y)iqr Interquartile range of pixel intensities for the region mask (Y) in color channel (X), 75th percentile - 25th percentile value
(X)_(Y)T20pct Mean intensity for the top 20% of pixel intensities for region mask (Y) in color channel (X). Useful in comparison with the next measurement to determine subcellular localization without a submask.
(X)_(Y)B80pct Mean intensity for the bottom 80% of pixel intensities for region mask (Y) in color channel (X). Useful in comparison with the previous measurement to determine subcellular localization without a submask.
(X)_(Y)M50pct Mean intensity for the middle 50% of pixel intensities for region mask (Y) in color channel (X)
(X)_cellint Integrated fluorescence of color channel (X) (mean pixel intensity x volume) for the whole cell (mother and any connected bud)
(Z)Raw Raw time series data output by "Time Series Analysis" for variable (Z)
(Z)Smooth Spline-smoothed raw time series data output by "Time Series Analysis" for variable (Z)
(Z)Whole Whole cell (mother + attached bud (Z)Rawsmooth) time series data output by "Time Series Analysis" for variable (Z)
(Z)Cont Whole cell time series data made continuous at divisions ("running total") output by "Time Series Analysis" for variable (Z)
(Z)WhSmooth Spline-smoothed (Z)Whole cell time series data output by "Time Series Analysis" for variable (Z)
(Z)ddt First time-derivative of spline-smoothed (Z)Whole cell time series data output by "Time Series Analysis" for variable (Z)
(Z)d2dt2 Second time-derivative of spline-smoothed (Z)Whole cell time series data output by "Time Series Analysis" for variable (Z)