Measurement Name
|
Description
|
Time |
Image frame number |
Area |
Total number of pixels comprising the region |
Volume |
(4/3)πabc, where a = ½ major axis length of region, b = ½ minor axis length, and c = b or ½ "Chamber height" (whichever is smaller) |
(X)_(Y)mean |
Mean pixel intensity for the region mask (Y) in color channel (X) |
(X)_(Y)median |
Median pixel intensity for the region mask (Y) in color channel (X) |
(X)_(Y)std |
Standard deviation of pixel intensities for the region mask (Y) in color channel (X) |
(X)_(Y)iqr |
Interquartile range of pixel intensities for the region mask (Y) in color channel (X), 75th percentile - 25th percentile value |
(X)_(Y)T20pct |
Mean intensity for the top 20% of pixel intensities for region mask (Y) in color channel (X). Useful in comparison with the next measurement to determine subcellular localization without a submask. |
(X)_(Y)B80pct |
Mean intensity for the bottom 80% of pixel intensities for region mask (Y) in color channel (X). Useful in comparison with the previous measurement to determine subcellular localization without a submask. |
(X)_(Y)M50pct |
Mean intensity for the middle 50% of pixel intensities for region mask (Y) in color channel (X) |
(X)_cellint |
Integrated fluorescence of color channel (X) (mean pixel intensity x volume) for the whole cell (mother and any connected bud) |
(Z)Raw |
Raw time series data output by "Time Series Analysis" for variable (Z) |
(Z)Smooth |
Spline-smoothed raw time series data output by "Time Series Analysis" for variable (Z) |
(Z)Whole |
Whole cell (mother + attached bud (Z)Rawsmooth) time series data output by "Time Series Analysis" for variable (Z) |
(Z)Cont |
Whole cell time series data made continuous at divisions ("running total") output by "Time Series Analysis" for variable (Z) |
(Z)WhSmooth |
Spline-smoothed (Z)Whole cell time series data output by "Time Series Analysis" for variable (Z) |
(Z)ddt |
First time-derivative of spline-smoothed (Z)Whole cell time series data output by "Time Series Analysis" for variable (Z) |
(Z)d2dt2 |
Second time-derivative of spline-smoothed (Z)Whole cell time series data output by "Time Series Analysis" for variable (Z) |