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. 2013 Aug 27;4(5):e00366-13. doi: 10.1128/mBio.00366-13

FIG 3 .

FIG 3 

Effect of leuO on aphA and tcpP expression. (A) Quantification of gene expression by qRT-PCR. The WT or ΔleuO strain grown under AKI conditions. (B) Effect of leuO on the expression of the indicated genes. The WT (pBAD18) and WT (pBAD18-leuO) strains were grown under AKI conditions for 3 h, when RNA was isolated and used for qRT-PCR. The means and SEM for three biological replicates are presented. *, P < 0.01 from a hypothetical value of 1.0. (C) TcpP Western blot of WT(pBAD18) and WT(pBAD18-leuO) grown under AKI conditions with 0.02% arabinose. Aliquots were collected at the indicated times and normalized by OD before analysis. (D) TcpP Western blot of the WT and ΔleuO strains grown under AKI conditions for the indicated times. The samples were processed as described above. Samples presented within panels C and D were run on the same gel and exposed to X-ray film for identical times. The resulting X-ray film images were then converted to gray-scale TIFF files, and the contrast and brightness were adjusted before being cropped to generate panels C and D.