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. 2013 Aug 27;4(5):e00366-13. doi: 10.1128/mBio.00366-13

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Copyright © 2013 Bina et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 7 — ToxR activates leuO expression. (A) DNA sequence of the leuO locus promoter region showing the location of the two ToxR-binding site consensus sequences in red. The ATG start codon for VC2486 is indicated by the green arrow. (B) Effect of toxRS on leuO expression in E. coli. E. coli containing a leuO-lux reporter (pJB906) and pBAD18-toxRS was grown in LB broth containing the indicated amounts of arabinose to induce toxRS expression. At the indicated time points, the relative luminescence (RLU) was determined and normalized by OD₆₀₀. The results are representative of three experiments ± SD.