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. 2013 Aug 27;4(5):e00476-13. doi: 10.1128/mBio.00476-13

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Copyright © 2013 Epp et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 2 — Arp2/3 is required for productive functioning of CME. Live-cell imaging data were analyzed for three additional quantitative elements to demonstrate that CME becomes nonproductive without Arp2/3. (A) The percentage of patches (n > 20) leaving the origin was defined in two ways; patches that disappeared (applied for the early, coat, Myo/WASP, and scission modules) and patches that moved more than 100 nm from the sites of first detection (applied for the actin module). In a few cases, Pan1 and End3 patches disappeared in arp2 cells, which likely correspond to patches moving out of the focal plane. (B) Track displacement length measures the distance between first and last patch detection, in arp2 cells corresponding to a maximum of 128 s. (C) Track duration refers to how long a patch was observed over a 2-min period. All graphs are expressed as means ± SD.