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. Author manuscript; available in PMC: 2014 Aug 6.
Published in final edited form as: Structure. 2013 Jul 25;21(8):1450–1459. doi: 10.1016/j.str.2013.06.015

Figure 4.

Figure 4

Mutational analysis of TopBP1 BRCT5 binding interface. (A) Replication stress-induced focus formation of wild-type and TopBP1 mutants. U2OS cells transfected with plasmids encoding SFB-tagged WT or mutants of TopBP1 were exposed to 2 mM HU for 3 hours. Cells were fixed and immunostained with anti-FLAG and anti-γ-H2AX antibodies. Bar: 10 µm. (B) FP binding studies of MDC1 FITC-labeled di-phospho-peptide with GST-fusion proteins of wild type BRCT5 and various missense variants. Triplicate data points are represented in graphs as mean ± SEM.