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. Author manuscript; available in PMC: 2014 Aug 6.

Published in final edited form as: Structure. 2013 Jul 25;21(8):1450–1459. doi: 10.1016/j.str.2013.06.015

Table 2.

Summary of Fluorescence Polarization Binding Experiments

Protein		Peptide	K_d (µM)
GST-BRCT5		FITC-GFIDSDTDVEEE-NH2	310 ± 70
		FITC-GFIDpSDTDVEEE-NH2	105 ± 9
		FITC-GFIDSDpTDVEEE-NH2	98 ± 9
		FITC-GFIDpSDpTDVEEE-NH2	28 ± 4
		FITC-GFIDpSDpTDDEEE-NH2	24 ± 3
GST-BRCT4/5		FITC-GFIDpSDpTDVEEE-NH2	27 ± 4
MBP-BRCT4/5		FITC-GFIDpSDpTDVEEE-NH2	82 ± 16
BRCT4/5		FITC-GFIDpSDpTDVEEE-NH2	94 ± 15
GST		FITC-GFIDpSDpTDVEEE-NH2	N.D.^a
GST-BRCT5 Mutants	S654A	FITC-GFIDpSDpTDVEEE-NH2	32 ± 3
	R681E/K682E	FITC-GFIDpSDpTDVEEE-NH2	280 ± 60
	K704A	FITC-GFIDpSDpTDVEEE-NH2	210 ± 50

^a

N.D. – not determined