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. 2013 Sep 3;4:257. doi: 10.3389/fimmu.2013.00257

Figure 9.

Figure 9

Effect of alanine substitutions in ECL2 of CX4-M1 on binding to gp120HxBc2 (A) (in the presence of sCD4) and its corresponding V3 loop peptide (V3HxBc2) (B), respectively. See Section “Materials and Methods” (direct ELISA) for experimental detail. Relative absorbances (Ar) were calculated according to the following formula: Ar = (Apeptide − Ablank)/(Ac − Ablank), in which “peptide” refers to the respective variant of CX4-M1, and “c” refers to CX4-M1. Error bars present deviations from the mean.