Table 3.
Sequences of V3 loop peptides.
| Peptide | Sequence |
|---|---|
| V3 LOOP PEPTIDES (WT) | |
| V3IIIB | Fluoa-Xb-G-X-CTRPNNNTRKKIRIQRGPGRAFVTIGKIGNMRQAHC-NH2 |
| V3HxBc2 | Yc-X-G-X-CTRPNNNTRKRIRIQRGPGRAFVTIGKIGNMRQAHC-NH2 |
| V3MN | Fluo-X-G-X-CTRPNYNKRKRIHI–GPGRAFYTTKNIIGTIRQAHC-NH2 |
| V3BAL | Yc-X-G-X-CTRPNNNTRKSIHI–GPGRALYTTGEIIGDIRQAHC-NH2 |
| V3ADA | Fluo-X-G-X-CTRPNNNTRKSIHI–GPGRAFYTTGEIIGDIRQAHC-NH2 |
| TRUNCATED VARIANTS of V3HxBc2 | |
| V3HxBc2 turn | Fluo-X-G-X––––––-CdQRGPGRACd–––––––-NH2 |
| V3HxBc2 turn/beta | Fluo-X-G-X––––-CdRIRIQRGPGRAFVTICd–––––-NH2 |
| V3HxBc2 Δbeta | Fluo-X-G-X-CTRPNNNTRK––QRGPGRA–-GKIGNMRQAHC–NH2 |
| ALANINE SCAN of V3HxBc2 | |
| V3HxBc2 R306A | Fluo-X-G-X-CTRPNNNTRKAIRIQRGPGRAFVTIGKIGNMRQAHC-NH2 |
| V3HxBc2 I307A | Fluo-X-G-X-CTRPNNNTRKRARIQRGPGRAFVTIGKIGNMRQAHC-NH2 |
| V3HxBc2 R308A | Fluo-X-G-X-CTRPNNNTRKRIAIQRGPGRAFVTIGKIGNMRQAHC-NH2 |
| V3HxBc2 I309A | Fluo-X-G-X-CTRPNNNTRKRIRAQRGPGRAFVTIGKIGNMRQAHC-NH2 |
| V3HxBc2 Q310A | Fluo-X-G-X-CTRPNNNTRKRIRIARGPGRAFVTIGKIGNMRQAHC-NH2 |
| V3HxBc2 R311A | Fluo-X-G-X-CTRPNNNTRKRIRIQAGPGRAFVTIGKIGNMRQAHC-NH2 |
| V3HxBc2 G312A | Fluo-X-G-X-CTRPNNNTRKRIRIQRAPGRAFVTIGKIGNMRQAHC-NH2 |
| V3HxBc2 P313A | Fluo-X-G-X-CTRPNNNTRKRIRIQRGAGRAFVTIGKIGNMRQAHC-NH2 |
| V3HxBc2 G314A | Fluo-X-G-X-CTRPNNNTRKRIRIQRGPARAFVTIGKIGNMRQAHC-NH2 |
| V3HxBc2 R315A | Fluo-X-G-X-CTRPNNNTRKRIRIQRGPGAAFVTIGKIGNMRQAHC-NH2 |
| V3HxBc2 F317A | Fluo-X-G-X-CTRPNNNTRKRIRIQRGPGRAAVTIGKIGNMRQAHC-NH2 |
| V3HxBc2 V318A | Fluo-X-G-X-CTRPNNNTRKRIRIQRGPGRAFATIGKIGNMRQAHC-NH2 |
| V3HxBc2 T319A | Fluo-X-G-X-CTRPNNNTRKRIRIQRGPGRAFVAIGKIGNMRQAHC-NH2 |
| V3HxBc2 I320A | Fluo-X-G-X-CTRPNNNTRKRIRIQRGPGRAFVTAGKIGNMRQAHC-NH2 |
| V3HxBc2 G321A | Fluo-X-G-X-CTRPNNNTRKRIRIQRGPGRAFVTIAKIGNMRQAHC-NH2 |
| V3HxBc2 K322A | Fluo-X-G-X-CTRPNNNTRKRIRIQRGPGRAFVTIGAIGNMRQAHC-NH2 |
| V3HxBc2 I323A | Fluo-X-G-X-CTRPNNNTRKRIRIQRGPGRAFVTIGKAGNMRQAHC-NH2 |
| V3HxBc2 G324A | Fluo-X-G-X-CTRPNNNTRKRIRIQRGPGRAFVTIGKIANMRQAHC-NH2 |
| V3HxBc2 N325A | Fluo-X-G-X-CTRPNNNTRKRIRIQRGPGRAFVTIGKIGAMRQAHC-NH2 |
| V3HxBc2 M326A | Fluo-X-G-X-CTRPNNNTRKRIRIQRGPGRAFVTIGKIGNARQAHC-NH2 |
aFluo, fluorescein; bX, ε-aminohexanoic acid (spacer); cY, fluorescein or biotin; dadditional cysteine residues forming a disulfide bridge.