Table 2.
Serum miRNA expression associated with 2-year survival in patients with advanced NSCLC from the study population recruited at the University of Texas MD Anderson Cancer Center between 2002 and 2009.
| Expression levela | Training set | Testing set | Combined set | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 2-year survival | 2-year survival | 2-year survival | ||||||||||||||
| N (No) | N (Yes) | Adjusted HRb (95% CI) |
P | MST | N (No) | N (Yes) | Adjusted HRb (95% CI) |
P | MST | N (No) | N (Yes) | Adjusted HRb (95% CI) |
P | MST | ||
| n% | n% | n% | n% | n% | n% | |||||||||||
| miR-16 | <0.71 | 83(97.7) | 2(2.4) | 1(reference) | 7.9 | 88(98.9) | 1(1.1) | 1(reference) | 9.0 | 171(98.3) | 3(1.7) | 1(reference) | 8.5 | |||
| >0.71 | 51(60.7) | 33(39.3) | 0.44(0.29–0.68) | 1.9×10−04 | 16.7 | 54(63.5) | 31(36.5) | 0.37(0.25–0.55) | 1.3×10−06 | 19.0 | 105(62.1) | 64(37.9) | 0.39(0.29–0.52) | 1.7×10−10 | 18.0 | |
| miR-30e-3p | <0.58 | 86(100) | 0(0) | 1(reference) | 8.3 | 89(98.9) | 1(1.1) | 1(reference) | 9.2 | 175(99.4) | 1(0.6) | 1(reference) | 8.7 | |||
| >0.58 | 48(56.5) | 37(43.5) | 0.45(0.29–0.69) | 3.1×10−04 | 20.4 | 54(62.8) | 32(37.2) | 0.38(0.25–0.58) | 5.6×10−06 | 19.0 | 102(59.7) | 69(40.4) | 0.4(0.30–0.54) | 7.8×10−10 | 19.8 | |
| miR-106a | <0.68 | 80(92.0) | 7(8.1) | 1(reference) | 7.6 | 83(98.8) | 1(1.2) | 1(reference) | 8.6 | 163(95.3) | 8(4.7) | 1(reference) | 8.5 | |||
| >0.68 | 56(64.4) | 31(35.6) | 0.57(0.39–0.85) | 5.7×10−03 | 15.7 | 60(65.2) | 32(34.8) | 0.40(0.28–0.59) | 2.8×10−06 | 16.0 | 116(64.8) | 63(35.2) | 0.47(0.36–0.61) | 2.0×10−08 | 16.0 | |
| miR-148b | <0.72 | 78(96.3) | 3(3.7) | 1(reference) | 7.8 | 78(96.3) | 3(3.7) | 1(reference) | 8.8 | 156(96.3) | 6(3.7) | 1(reference) | 8.3 | |||
| >0.72 | 51(63.0) | 30(37.0) | 0.49(0.33–0.74) | 5.9×10−04 | 16.4 | 57(67.1) | 28(32.9) | 0.51(0.35–0.75) | 5.3×10−04 | 14.4 | 108(65.1) | 58(34.9) | 0.5(0.38–0.65) | 3.4×10−07 | 15.5 | |
| miR-26a | <0.68 | 78(89.7) | 9(10.3) | 1(reference) | 8.5 | 77(96.3) | 3(3.8) | 1(reference) | 9.0 | 155(92.8) | 12(7.2) | 1(reference) | 8.7 | |||
| >0.68 | 57(66.3) | 29(33.7) | 0.65(0.44–0.95) | 2.8×10−02 | 15.7 | 65(68.4) | 30(31.6) | 0.45(0.30–0.66) | 5.8×10−05 | 15.5 | 122(67.4) | 59(32.6) | 0.51(0.39–0.67) | 7.5×10−07 | 15.7 | |
| miR-27a | <0.67 | 81(93.1) | 6(6.9) | 1(reference) | 8.6 | 79(97.5) | 2(2.5) | 1(reference) | 9.0 | 160(95.2) | 8(4.8) | 1(reference) | 8.7 | |||
| >0.67 | 55(64.0) | 31(36.1) | 0.59(0.4–0.87) | 7.3×10−03 | 16.4 | 64(67.4) | 31(32.6) | 0.47(0.32–0.71) | 2.3×10−04 | 16.0 | 119(65.8) | 62(34.3) | 0.51(0.39–0.67) | 9.3×10−07 | 16.2 | |
| miR-106b | <0.70 | 81(93.1) | 6(6.9) | 1(reference) | 7.2 | 85(95.5) | 4(4.5) | 1(reference) | 8.9 | 166(94.3) | 10(5.7) | 1(reference) | 8.3 | |||
| >0.70 | 55(64.0) | 31(36.1) | 0.52(0.35–0.77) | 1.1×10−03 | 16.4 | 58(66.7) | 29(33.3) | 0.5(0.34–0.72) | 2.8×10−04 | 15.5 | 113(65.3) | 60(34.7) | 0.53(0.41–0.68) | 9.4×10−07 | 16.0 | |
| miR-301a | <1.16 | 71(92.2) | 6(7.8) | 1(reference) | 7.6 | 73(93.6) | 5(6.4) | 1(reference) | 8.3 | 144(92.9) | 11(7.1) | 1(reference) | 7.9 | |||
| >1.16 | 50(65.8) | 26(34.2) | 0.53(0.36–0.8) | 2.1×10−03 | 14.4 | 51(66.2) | 26(33.8) | 0.54(0.35–0.82) | 3.7×10−03 | 16.0 | 101(66.0) | 52(34.0) | 0.53(0.40–0.70) | 6.7×10−06 | 15.8 | |
| miR-27b | <0.72 | 79(91.9) | 7(8.1) | 1(reference) | 7.1 | 75(93.8) | 5(6.3) | 1(reference) | 9.0 | 154(92.8) | 12(7.2) | 1(reference) | 8.2 | |||
| >0.72 | 56(65.9) | 29(34.1) | 0.53(0.36–0.79) | 1.6×10−03 | 14.8 | 66(74.2) | 23(25.8) | 0.66(0.46–0.93) | 1.9×10−02 | 11.1 | 122(70.1) | 52(29.9) | 0.58(0.45–0.75) | 2.2×10−05 | 13.2 | |
| miR-152 | <0.68 | 77(91.7) | 7(8.3) | 1(reference) | 7.8 | 82(92.1) | 7(7.9) | 1(reference) | 8.6 | 159(91.9) | 14(8.1) | 1(reference) | 8.4 | |||
| >0.68 | 55(66.3) | 28(33.7) | 0.6(0.41–0.87) | 7.2×10−03 | 14.4 | 56(70) | 24(30.0) | 0.60(0.42–0.86) | 5.3×10−03 | 12.7 | 111(68.1) | 52(31.9) | 0.59(0.46–0.75) | 3.3×10−05 | 14.1 | |
| miR-20a | <0.67 | 79(92.9) | 6(7.1) | 1(reference) | 7.8 | 76(92.7) | 6(7.3) | 1(reference) | 9.0 | 155(92.8) | 12(7.2) | 1(reference) | 8.5 | |||
| >0.67 | 55(65.5) | 29(34.5) | 0.63(0.42–0.95) | 2.7×10−02 | 14.3 | 66(71.0) | 27(29.0) | 0.58(0.41–0.83) | 2.5×10−03 | 12.9 | 121(68.4) | 56(31.6) | 0.62(0.48–0.79) | 1.5×10−04 | 14.1 | |
| miR-30a-5p | <0.61 | 75(89.3) | 9(10.7) | 1(reference) | 8.7 | 76(90.5) | 8(9.5) | 1(reference) | 8.6 | 151(89.9) | 17(10.1) | 1(reference) | 8.7 | |||
| >0.61 | 55(65.5) | 29(34.5) | 0.69(0.48–1.01) | 5.5×10−02 | 9.6 | 66(72.5) | 25(27.5) | 0.58(0.41–0.83) | 2.6×10−03 | 11.6 | 121(69.1) | 54(30.9) | 0.63(0.49–0.81) | 3.2×10−04 | 11.5 | |
| miR-148a | <0.74 | 79(92.9) | 6(7.1) | 1(reference) | 8.2 | 80(90.9) | 8(9.1) | 1(reference) | 9.6 | 159(91.9) | 14(8.1) | 1(reference) | 8.8 | |||
| >0.74 | 55(64.7) | 30(35.3) | 0.57(0.39–0.84) | 3.8×10−03 | 14.3 | 59(70.2) | 25(29.8) | 0.72(0.49–1.04) | 8.3×10−02 | 12.9 | 114(67.5) | 55(32.5) | 0.63(0.49–0.81) | 4.1×10−04 | 14.1 | |
| miR-145 | <0.62 | 77(91.7) | 7(8.3) | 1(reference) | 8.2 | 73(90.1) | 8(9.9) | 1(reference) | 9.6 | 150(90.9) | 15(9.1) | 1(reference) | 8.8 | |||
| >0.62 | 54(65.1) | 29(34.9) | 0.62(0.42–0.92) | 1.7×10−02 | 15.7 | 67(72.0) | 26(28.0) | 0.70(0.49–0.99) | 4.3×10−02 | 12.4 | 121(68.8) | 55(31.3) | 0.64(0.49–0.82) | 4.4×10−04 | 12.7 | |
| miR-92a | <0.63 | 77(88.5) | 10(11.5) | 1(reference) | 8.6 | 73(91.3) | 7(8.8) | 1(reference) | 8.9 | 150(89.8) | 17(10.2) | 1(reference) | 8.7 | |||
| >0.63 | 59(67.8) | 28(32.2) | 0.70(0.48–1.04) | 7.6×10−02 | 14.1 | 70(72.9) | 26(27.1) | 0.66(0.47–0.92) | 1.6×10−02 | 11.1 | 129(70.5) | 54(29.5) | 0.67(0.53–0.86) | 1.6×10−03 | 12.4 | |
| let-7c | <0.66 | 74(89.2) | 9(10.8) | 1(reference) | 8.4 | 66(88.0) | 9(12.0) | 1(reference) | 9.2 | 140(88.6) | 18(11.4) | 1(reference) | 8.6 | |||
| >0.66 | 56(68.3) | 26(31.7) | 0.65(0.44–0.94) | 2.4×10−02 | 14.3 | 68(73.9) | 24(26.1) | 0.72(0.50–1.04) | 7.7×10−02 | 11.6 | 124(71.3) | 50(28.7) | 0.68(0.53–0.87) | 2.6×10−03 | 13.4 | |
| let-7b | <0.60 | 78(90.7) | 8(9.3) | 1(reference) | 8.6 | 89(92.7) | 7(7.3) | 1(reference) | 9.3 | 167(91.8) | 15(8.2) | 1(reference) | 9.0 | |||
| >0.60 | 55(64.7) | 30(35.3) | 0.65(0.44–0.97) | 3.5×10−02 | 11.3 | 53(67.1) | 26(32.9) | 0.67(0.46–0.98) | 3.9×10−02 | 13.7 | 108(65.9) | 56(34.2) | 0.69(0.53–0.89) | 4.5×10−03 | 12.7 | |
a
Expression levels were calculated using the 2−ΔΔCt method. ΔCt was defined as the raw Ct value for a specific miRNA (or spike-in control miRNAs) in an individual sample minus the mean raw Ct value of this miRNA (or spike-in control miRNAs) in all the tested samples. ΔΔCt was defined as the difference between ΔCt of the specific miRNA and ΔCt of the spike-in control miRNAs.
b
Adjusted for age, sex, smoking status, clinical stage, and treatment regime