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. Author manuscript; available in PMC: 2013 Sep 3.
Published in final edited form as: Growth Factors. 2008 Aug;26(4):226–237. doi: 10.1080/08977190802277880

Figure 2.

Figure 2

rhBMP-2 and rhBMP-4 comparably induce ALP activity but not mineralization in DEX-inhibited cultures. MC3T3-E1 cultures were treated with 1 μM DEX commencing on day 3 and with rhBMP-2 or rhBMP-4, 2 days later. (A) ALP activity was measured biochemically 24 h after administration of rhBMP-2 or rhBMP-4 at concentrations between 0.1–100 ng/ml. ALP activity was corrected for DNA content, and the fold stimulation was calculated relative to cultures not treated with BMP. Mean ± SD; n = 3. (B) Top, schematic illustration of the experimental design. Cultures were treated chronically with 1 μM DEX and exposed to 100 ng/ml rhBMP-2 or -4 for a brief 10-h period on day 5. Bottom, Alizarin Red staining of calcium deposition on day 14. Stained plate is representative of three experiments with similar results.