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. Author manuscript; available in PMC: 2013 Sep 3.
Published in final edited form as: Growth Factors. 2008 Aug;26(4):226–237. doi: 10.1080/08977190802277880

Table I.

Primers and conditions used for PCR.

Gene Accession no. Primers Annealing temp.°C Cycles Amplicon size (bp)
Bmp2, exon 3 NM_007553 F: 5′-AGT TCT GTC CCC AGT GAC GAG TTT
R: 5′-GTA CAA CAT GGA GAT TGC GCT GAG
63 35 708
Bmp4, exon 3 NM_007554 F: 5′-GAG AAC ATC CCA GGG ACC AGT
R: 5′-TGT GAT GAG GTG TCC AGG AA
63 35 212
Bmp2 NM_007553 F: 5′-GAC GGA CTG CGG TCT CCT AAA G
R: 5′-TCT GCA GAT GTG AGA AAC TCG TCA
64 40 496
Bmp4 NM_007554 F: 5′-GCG CCG TCA TTC CGG ATT AC
R: 5′-CAT TGT GAT GGA CTA GTC TG
63 32 402
L10A U12403 F: 5′-CGC CGC AAG TTT CTG GAG AC
R: 5′-CTT GCC AGC CTT GTT TAG GC
61 29 327