Table I.
Gene | Accession no. | Primers | Annealing temp.°C | Cycles | Amplicon size (bp) |
---|---|---|---|---|---|
Bmp2, exon 3 | NM_007553 | F: 5′-AGT TCT GTC CCC AGT GAC GAG TTT R: 5′-GTA CAA CAT GGA GAT TGC GCT GAG |
63 | 35 | 708 |
Bmp4, exon 3 | NM_007554 | F: 5′-GAG AAC ATC CCA GGG ACC AGT R: 5′-TGT GAT GAG GTG TCC AGG AA |
63 | 35 | 212 |
Bmp2 | NM_007553 | F: 5′-GAC GGA CTG CGG TCT CCT AAA G R: 5′-TCT GCA GAT GTG AGA AAC TCG TCA |
64 | 40 | 496 |
Bmp4 | NM_007554 | F: 5′-GCG CCG TCA TTC CGG ATT AC R: 5′-CAT TGT GAT GGA CTA GTC TG |
63 | 32 | 402 |
L10A | U12403 | F: 5′-CGC CGC AAG TTT CTG GAG AC R: 5′-CTT GCC AGC CTT GTT TAG GC |
61 | 29 | 327 |