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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Dev Dyn. 2012 Sep 17;241(11):1695–1707. doi: 10.1002/dvdy.23857

FIGURE 4. Decreasing ORC5 levels suppresses de2f1-dsRNA effects on S-phase progression.

FIGURE 4

4A) Immunohistochemical staining of BrdU (red) of ptc-GAL4-driven de2f1-dsRNA expressing 3rd-instar wing discs showed a significant reduction in BrdU positive cells (b, b’) when compared to wild-type discs (a, a’). Knock-down of ORC5 at the same time restored the normal BrdU pattern (c, c’) and expression of Orc5-dsRNA alone had no detectable effect on S-phase progression (d, d’). The ptc-expressing stripe is indicated by expression of the UAS-GFP transgene (a”-d”).

4B) BrdU incorporation in red in third instar larvae eye discs. GMR-GAL4 driven expression of de2f1-dsRNA broadens the stripe of S-phase cells and this effect is suppressed by co-expression of Orc5-dsRNA