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. Author manuscript; available in PMC: 2013 Sep 3.
Published in final edited form as: Structure. 2011 Mar 9;19(3):418–429. doi: 10.1016/j.str.2010.12.015

Figure 6. IFI16 HIN-B Domain Enhances p53-DNA Binding by Associating with p53 Core Domain.

Figure 6

(A) Polyacrylamide EMSA reactions without p53 (82–360) (lanes 1–5) or with constant amount (2 μM) of p53 (lanes 6–10), in the presence of increasing protein concentration (0, 10, 20, 40, 80 μM) of HIN-B.

(B) GST-pull-down of p53 and HIN-B domain. An equimolar mixture of His-tagged p53 (82–360) and GST-tagged IFI16 HIN-B, GST-tagged IFI16 HIN-A, or GST protein alone was mixed with glutathione-sepharose (L). After washing (Washes 1–3), protein was eluted (E1, E2) with reduced glutathione and visualized by Coomassie blue staining.

(C) Agarose EMSA of constant amount (0.2 μM) of p53(82–393) in the presence of increasing protein concentration (0, 0.07, 0.2 μM) of full-length IFI16.

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