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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Traffic. 2012 Oct 11;13(12):1653–1666. doi: 10.1111/tra.12009

Figure 3.

Figure 3

CLSEM analysis of actin at cell-cell contacts in control and clathrin depleted cells. Jeg3 cells transfected with Cy3-tagged control (a) or anti-clathrin siRNAs (b) were permeabilized and labeled for actin (red). Cells were first imaged with an epifluorescence microscope (Actin and phase panels in a and b) and then processed for scanning electron microscopy (SEM). The ultrastructure of the cytoskeleton in control and clathrin knocked down cells was compared. In both a and b, panels 2 and 3 represent higher magnifications of the boxed areas in panel 1. Full and empty arrowheads point at actin structures at cell-cell contacts.