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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: Exp Cell Res. 2013 Jun 25;319(14):2275–2281. doi: 10.1016/j.yexcr.2013.06.010

Figure 4. Interaction of TTC30B and KIF17 with IFT52 and IFT57 in cells.

Figure 4

(A) HEK293 cells were transfected with expression vectors for AU1-tagged TTC30B or HA-tagged KIF17 and FLAG tagged-IFT52 or FLAG-tagged IFT57 as indicated. At 20 hours after transfection, whole cell extracts were prepared. (A) Samples were analyzed by denaturing gel electrophoresis and immunoblotting with antibody to AU1 to detect the input expression of TTC30B. (B) To detect interaction of TTC30B with IFT52 or IFT57, FLAG-tagged proteins were isolated by immunoprecipitation with FLAG antibody and the immunoprecipitate was analyzed by gel electrophoresis and immunoblotting with AU1 antibody to detect co-immunoprecipitated AU1-TTC30B. (C) Input expression of HA-KIF17 was detected by gel electrophoresis and immunoblotting with HA antibody. (D) Interaction of KIF17 with the IFT complex was analyzed by immunoprecipitation with FLAG antibody and the immunoprecipitate was analyzed by gel electrophoresis and immunoblotting with HA antibody to detect co-immunoprecipitated HA-KIF17.