Fig. 2.

Comparison of the application of the ¹H/¹⁵N HETCOR pulse sequences to the membrane-bound form of Pf1 coat protein in magnetically aligned bilayers (top panels) and a NAL single crystal (bottom panels). (A and C) The experimental spectra were obtained using the conventional (Fig. 1A) HETCOR pulse sequence. (B and D) The experimental spectra were obtained using the new version (Fig. 1B) of the HETCOR pulse sequence. A one-dimensional spectral slice from the t1 dimension is shown on the right side of each two-dimensional spectrum, and the signal selected for each slice is labeled with an arrow on the respective two-dimensional spectra. The half brace designates the resonance peaks of I39 and V35. No apodization functions were applied in processing the t1 dimension, and Fig. 3 shows the time domain evolution of signals that contribute to spectra A and B. The resonance assignments for all peaks in spectrum B are shown in Fig. 6.