Fig. 7.

Two-dimensional SLF spectrum (A) and selective planes from three-dimensional HETCOR/SLF spectrum (B) obtained from the uniformly ¹⁵N labeled mercury transport protein MerFm in magnetically aligned bilayers. More than 50 residues are heavily overlapped in the helical region (¹H–¹⁵N dipolar couplings > 1 kHz) make it a challenging target for structure determination with two-dimensional experiments. The selected HETCOR planes from a three-dimensional experiment display resolution similar to that observed in the membrane-bound form of the Pf1 coat protein in magnetically aligned bilayers in Fig. 6.