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CD spectra of BmGlvs in the presence of smooth LPS, Ra-, Rc- and Re-LPS and lipid A at pH 5.0. Purified BmGlv1–4 were diluted to 0.15 mg/ml in 10 mM phosphate buffer at pH 5.0 in the presence or absence of smooth LPS, Ra-, Rc- and Re-LPS or monophosphoryl lipid A (w/w= 1:1) and CD spectra were recorded on a Jasco-810 spectropolarimeter at 25 °C. Each CD spectrum was obtained after subtracting the signal from protein-free solution.
