Fig. 7.

Dose-dependent activity of BmGlvs against E. coli D21 and D21f2 mutant strains. Mid-log phase E. coli D21 and D21f2 cells were diluted to OD600 = 0.4 in 10 mM phosphate, 100 mM NaCl, pH 5.0, and then incubated with increasing concentrations of purified BmGlvs (final concentration of 0.4,2 and 10 µM)or buffer (Control) in 96-well plates with 220 rpm shaking at 37 °C OD₆₀0 was recorded every hour after incubation. The points represent the mean of four individual measurements ± SEM.