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. Author manuscript; available in PMC: 2013 Sep 3.
Published in final edited form as: Methods Mol Biol. 2013;962:95–111. doi: 10.1007/978-1-62703-236-0_8

Fig. 5.

Fig. 5

The optimal concentration of the free probe for FP assay is determined by varying the concentration of the free probe and identify the lowest concentration that starts to give a stable polarization signal.