Fig. 11.

Two-dimensional ¹⁵N/ ¹³C HETCOR spectrum of the magnetically aligned sample of Pf1 bacteriophage with the coat protein labeled with both ¹³C and ¹⁵ N. The spectrum was acquired at 0 °C. Thirty two scans of 80 complex points were acquired in the indirect dimension with a spectral width of 30 kHz. The recycle delay was 6 s. The data were zero filled to 1K points and multiplied by a sine bell window function in both dimensions prior to double Fourier transformation. The carrier frequency for ¹³C irradiation was in the center of the spectrum, between the aliphatic and carbonyl resonance regions, and the double cross-polarization was optimized for both types of resonances.