Figure 2.

Maximum-likelihood (ML) phylogenetic tree of chaperonin-like sequences including canonical CCT subunits and CL-BBS proteins, rooted with the thermosome alpha-subunit sequence from Thermoplasma acidophilum (PDB code 1A6D). TCP1 is synonymous with CCT1. MKKS is synonymous with BBS6. Names of CL-BBS sequences identified in this study from non-vertebrate Metazoa are indicated in red and those from Oomycetes in blue. Sequences representing all major eukaryotic groups are included for CCT8. Values associated with branches indicate levels of support measured by aLTR (black) and are compared at relevant branches with posterior probabilities (green) of a Bayesian tree (Supplementary Figure S1) and with bootstrap values (blue, 1000 replicates) of a neighbor-joining tree based on pairwise distances estimated with the JTT matrix and gamma distributed rates with parameter a = 2.212 (see Methods and Supplementary Figure S2). BBS6 and BBS12 are sister groups in the Bayesian and NJ trees (with low support). Species names are abbreviated as indicated in Table 1. Other species abbreviations are: Dr, Danio rerio; Gg, Gallus gallus; Hs, Homo sapiens; Md, Monodelphis domestica; Oa, Ornithorhynchus anatinus; Xl, Xenopus laevis; Xt, Xenopus tropicalis.