Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep 2;202(5):747–763. doi: 10.1083/jcb.201303066

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Huntwork-Rodriguez et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — DLK protein levels and molecular weight increase in response to NGF withdrawal from embryonic sensory neurons. (A) Increase in DLK abundance and molecular weight in response to NGF withdrawal from cultured embryonic DRGs. c-Jun phosphorylation (p-c-Jun) occurs as a downstream consequence of DLK and JNK activation. (B) Ratio of DLK protein quantity in −NGF vs. +NGF samples shown in A. Ratio = 2.12 ± 0.127. *, P = 0.02 by Mann-Whitney U test comparing the mean ratio to 1. Error bar represents SD. (C) Table showing molecular weight of DLK in kilodaltons (mean and SD) in +NGF and –NGF samples shown in A. *, P = 0.03 by Mann-Whitney U test comparing the molecular weights of DLK in the two conditions. (D) Lambda protein phosphatase (λpp) treatment of DRG lysates equalizes DLK molecular weight in −NGF and +NGF conditions. (E) Difference in mobility of DLK in SDS-PAGE with two different running buffers. MOPS buffer: SDS-PAGE running solution containing MOPS as a buffer. MES buffer: SDS-PAGE running solution containing MES as a buffer. MW (kD): molecular weight in kilodaltons.