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. 2012 Apr 16;1:10.3402/jev.v1i0.18397. doi: 10.3402/jev.v1i0.18397

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© 2012 Angélique Bobrie et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1 — Selection of a shRNA for specific inhibition of mouse Rab27a. A, Effect of 2 different shRNA sequences specific for Rab27a (sh27a2 and sh27a4) on Rab27a and Rab27b expression, measured by qRT-PCR. In each experiment, Rab27 expression in shRab27-expressing cells is compared to expression in cells expressing a control shRNA (Scr). Individual results of 3 (sh27a4) or 7 (sh27a2) experiments are shown. Both sh27a2 and sh27a4 significantly decrease Rab27a expression, but sh27a4 also increases Rab27b expression. ***p < 0.01, 1-way ANOVA, Dunnett's post-test. B, Stable expression of sh27a2 induces downregulation of Rab27a at the protein level, as shown by Western blotting on total cell lysate (left panel). Actin is shown as loading control. Individual values of arbitrary units of Rab27a/actin band intensity in 2 independent experiments is shown (right panel). In each experiment, arbitrary units obtained for Scr cells are considered as 100%.