Fig. 2.

Inhibition of Rab27a decreases secretion of a subset of exosomal markers. A, Western blot characterization of exosomes (Exo) secreted by the same number of control (Scr) or shRab27a-expressing 4T1 cells (sh27a), i.e. corresponding to 2–3 µg of total proteins. 30 µg of total cell lysates (cell) were analysed in parallel. Six different exosomal markers (CD63, Alix, Tsg101, Hsc70, CD9 and Mfge8) and a negative control (gp96) are shown. B, Quantification of the amount of each marker in exosomes obtained from shRab27a-expressing cells, as compared to control cells (individual results from 5 independent experiments are shown). *p < 0.05, **p < 0.01, paired t-test.