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. 2013 May 6;2:10.3402/jev.v2i0.20181. doi: 10.3402/jev.v2i0.20181

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© 2013 Erika Bartolini et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4 — Antibodies directed against CM rHtrA-OMV neutralize Chlamydia infectivity in vitro. CM EBs (left panel) and CT EBs (right panel) were opsonized with sera directed against CM rHtrA, CM rHtrA-OMV, “empty” OMV at different dilutions and used to infect LLC-MK2 cell monolayers, in comparison with EBs treated with corresponding dilutions of pre-immune sera. As controls, EBs were also incubated with anti-CM rMOMP and anti-CT rMOMP antibodies. The antiserum neutralization activity was determined by measuring the reduction of the number of inclusions generated by antibody-opsonized EBs after correction for background inhibition observed with pre-immune sera. The graphs present the percent neutralization averaged from triplicate experiments, with standard deviations.