Table I.

2012 ISEV Research Seminar discussion topics

Round table	Topic	Description
1	EV isolation	Pelleting, filtration, density gradient, controls
2	RNA isolation	Comparison of different isolation protocols
3	evRNA analysis	Hybridization, qPCR, RNase treatment
4	evRNA analysis and bioinformatics	Deep sequencing, analysis pipelines, recent developments
5	RNA loading and engineered vesicles	Electroporation, loading control, cellular production, targeting
6	Analysis of functional evRNA transfer	Reporter systems, physiological read-out systems