Fig. 3.

Adenine inhibits IP₃-mediated increase in cytosolic [Ca²⁺]_i. a Analysis of the flurophore emission in response to uncaging IP₃ shows a rapid increase in cytosolic [Ca²⁺]_i over approximately 5 ms, with a gradual decline over the next 40 ms, as seen in the darker line from a control T-6 cell. Pretreatment of T-6 cells with adenine inhibits the increase in cytosolic [Ca²⁺]_i in response to uncaging IP₃ as seen in the lighter line. b Summation of data from control and adenine-treated T-6 cells shows an inhibition of the increase in cytosolic Ca²⁺ in response to uncaging IP₃ (* P < 0.05). Experiment repeated four times