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. 2013 Sep 3;11(9):e1001649. doi: 10.1371/journal.pbio.1001649

Figure 1. Direct interactions of AT1G75060 (AFR1) and AT1G19330 (AFR2) with AtSAP18 and HDA19 proteins.

Figure 1

(A) Interactions of AtSAP18 with AT1G75060 and AT1G19330 in yeast. The indicated proteins of full-length were fused with the GAL4-BD or AD domain. Yeast cells harboring the fusion proteins, BD and/or AD (as indicated), were grown on selective synthetic defined media lacking of Trp, Leu, and His. (B) BiFC analysis of the interactions of AtSAP18 with AT1G75060 and AT1G19330 in onion epidermal cells. Onion epidermal cells were co-transformed transiently by a pair of plasmid, as indicated, via biolistic gene bombardment. Yellowish-green signals indicate physical associations of paired proteins in the nuclei. Blue fluorescence from a DAPI (4′,6-diamidino-2-phenylindole) staining indicates a nucleus. Bar = 20 µm. (C) Interactions of HDA19 with AT1G75060 and AT1G19330 in yeast. The indicated full-length proteins were fused with the GAL4-BD or AD domain. Yeast cells were grown on selective synthetic defined media lacking of Trp, Leu, and His. (D) BiFC analysis of the interactions of HDA19 with AT1G75060 and AT1G19330 in onion epidermal cells. Bar = 20 µm.