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. 2013 Sep 3;8(9):e73781. doi: 10.1371/journal.pone.0073781

Table 2. Mortality parameter estimates from a two-phase Gompertz fit to daily mortality of adult Calliphora stygia maintained at different ambient temperatures.

Temperature(°C) Initial mortality%(% mortality per day) Duration of 1stphase of ageing(days) Rate of 1st-phaseageing (% change inmortality rate per day) Rate of 2nd-phaseageing (% change inmortality rate per day) Single vs.two-phaseGompertz (R2)
12 0.6±0.4a 83±6a 0.8±0.3a# 4.0±0.3a 0.72 vs. 0.81
15 0.6±0.1a 48±5b −0.6±0.6a# 3.5±0.2b 0.78 vs. 0.84
20 0.9±0.7a 61±4ab −0.4±0.5a# 5.7±0.6c 0.49 vs. 0.68
25 1.1±0.2a 55±4b 2.4±0.4b 9.3±1.8d 0.66 vs. 0.74
29 0.6±0.3a 17±3c 16.9±3.4c 3.0±0.6e 0.64 vs. 0.77
34 0.6±0.1a 10±1c 33.3±5.4d 5.5±1.1abc 0.72 vs. 0.88
Transfer from 29°C to 15°C
14 days 0.7±0.3 83±4C 0.0±0.0#B 4.6±0.4B 0.50 vs. 0.71
28 days 0.9±0.2 72±8AC 1.5±0.4#C 6.0±1.4C 0.54 vs. 0.63
Transfer from 15°C to 29°C
14 days 0.8±0.4 na na 5.15±0.5BC 0.64 vs. na
28 days 0.3±0.3 21±5B 10.8±2.0D 4.81±0.9BC 0.79 vs. 0.82
%

Initial mortality, calculated as the average mortality over the first five-days, was invariant between constant temperatures as compared by Kruskal- Wallis ANOVA (K-W = 0.65, df = 5, P>0.05).

Daily mortality data were fitted by both single linear regression and segmental linear regression, the latter giving two phases of ageing (1st and 2nd) and a time point at which the two phases intersect (length of 1st period). For each temperature treatment these two equations were compared by an AICc comparison to determine the most likely model to have generated the data, R2 values are given for each fit. In the temperature transfer experiment, mortality curves were applied immediately following the transfer.

For each mortality parameter, populations are compared between temperature pairs by an F-test (P<0.01). Values that share the same letter are not significantly different (lowercase letters compare constant temperatures, uppercase letters are used to compare between temperature transfer populations).

#

The 1st phase of ageing was also tested to determine whether the rate of ageing (i.e. the slope of the line) was significantly different from zero. Slopes that were not significantly different from zero are denoted by #.