Table 1. Oligonucleotides used in this study.
Oligonucleotide | Sequence (5’–3’) |
---|---|
1 | GACG A A T T CTACAATTTCAGGTGGCAC |
2 | GGTG A A T T CGTAAACTTGGTCTGACAG |
3 | CCGATTAC A T A T GGAAGCAGTAGCTTATTC |
4 | GAC T C G A GATCATCAGATTCAACCTCG |
5 | TTCAG G A T C CAGGATTATGTGTATTGCG |
6 | CCGCA A G C T TTTAAGTTTCTGACCCTTTCC |
7 | GAGTA C T A G TGAAAAAGCAGGATTTGAGG |
8 | CCAAG G A T C CGAATAAGCTACTGCTTCC |
9 | CGGTCG G A T C CAATAAAGATAATCATC |
10 | ATTCG G A T C CTTAATAGTGATCTTTTGCAG |
11 | CGGGA C T A G TTAGAAATAAATAAGGGGT |
12 | CAAAAAGAGATTACGACTCTATGCAAGAAACG |
13 | CGTTTCTTGCATAGAGTCGTAATCTTTTTTG |
14 | CGCGGG A A T T CTAGAAATAAATAAGGGGT |
15 | GCACTAAATCATCACTTTCGGGAAAG |
16 | GAGTG A A T T CGAAAAAGCAGGATTTGAGG |
17 | ATCG G A T C CGTAATACGCGTAAC |
18 | CCGCA A G C T TGCTCATGCCAATAAAGATAATC |
19 | [BTN]AGCAACTAAAGCAGAAGTACGGC |
20 | TCATATAACTACGTAAATTTTGGCGG |
21 | [BTN]TTCCGCCAAAATTTACGTAGTTA |
22 | TTGCATAGAATCATAATCTCTTTTTGA |
Restriction sites or introduced mutations are underlined.