| pBBRlux |
Vector for generating transcriptional fusion to lux, Cmr
|
[32] |
| pBBRlux-amp |
Vector for generating transcriptional fusion to lux, bla gene was amplified with primers 1/2 and cloned into EcoRI site within cat gene |
This study |
| pET22b(+) |
IPTG-inducible expression vector allowing fusion of C-terminal His6 tag to the target protein, AmpR
|
Novagen |
| pET22axe |
axe gene amplified with primers 3/4, digested with NdeI-XhoI and cloned between equivalent sites in pET22(+) |
This study |
| pET22at_axe-txe |
at_axe-txe fragment amplified with primers 5/6, digested with BamHI-HindIII and cloned between equivalent sites in pET22(+) |
This study |
| pluxat |
p
at promoter-operator region amplified with primers 7/8 (209 bp), digested with SpeI-BamHI and cloned between equivalent sites in pBBRlux-amp |
This study |
| pluxat_axe |
fragment containing p
at promoter-operator region and axe gene amplified with primers 7/9 (497 bp), digested with SpeI-BamHI and cloned between equivalent sites in pBBRlux-amp |
This study |
| pluxat_axe-txe |
fragment containing p
at promoter-operator region and axe-txe genes amplified with primers 7/10 (708 bp), digested with SpeI-BamHI and cloned between equivalent sites in pBBRlux-amp |
This study |
| pluxaxe |
p
axe promoter-operator region amplified with primers 9/11 (353 bp), digested with SpeI-BamHI and cloned between equivalent sites in pBBRlux-amp |
This study |
| pluxaxemut |
p
axe promoter-operator region with mutated -10 box (site-directed mutagenesis with primers 12/13) amplified with primers 9/11 (353 bp), digested with SpeI-BamHI and cloned between equivalent sites in pBBRlux-amp |
This study |
| pluxaxe-txeW5C |
axe-txe genes with amino acid change in Txe protein (W5C) amplified with primers 10/11 (564 bp), digested with SpeI-BamHI and cloned between equivalent sites in pBBRlux-amp |
This study |
| pREG531 |
pFH450 derivative plasmid containing axe-txe cassette, used for amplifications of this module and plasmid stability tests, Cmr
|
[24] |
| pREGpaxemut |
pREG531 derivative with p
axe promoter-operator region mutated in -10 box (site-directed mutagenesis with primers 12/13) |
This study |
| pREGΔaxetxe |
pREG531 derivative, where axe-txe cassette was cut out with enzymes KpnI and SpeI and vector was religated |
This study |
| pTE103 |
Vector for generating transcription templates, contains the multicloning site from pUC8 placed upstream from a bacteriophage T7 transcriptional terminator, AmpR
|
[33] |
| pTEat_axetxe |
fragment containing p
at promoter-operator region and axe-txe genes amplified with primers 6/16, digested with EcoRI-HindIII and cloned between equivalent sites in pTE103 |
This study |
| pTEat_axetxemut |
fragment containing p
at promoter-operator region and axe-txe genes with mutated -10 box in p
axe promoter amplified with primers 6/16, digested with EcoRI-HindIII and cloned between equivalent sites in pTE103 |
This study |
| pTEaxetxeW5C |
axe-txe genes with amino acid change in Txe protein (W5C) amplified with primers 6/14, digested with EcoRI-HindIII and cloned between equivalent sites in pTE103 |
This study |
| pTEaxe |
axe and first 60 bp of txe genes amplified with primers 14/18, digested with EcoRI-HindIII and cloned between equivalent sites in pTE103 |
This study |
| pTEat_axe-txe_ter |
fragment containing p
at promoter-operator region and axe-txe genes along with the terminator region downstream of txe, amplified with primers 16/17, digested with EcoRI-BamHI and cloned between equivalent sites in pTE103 |
This study |
