Skip to main content
. 2013 Sep 3;8(9):e73633. doi: 10.1371/journal.pone.0073633

Figure 1. Schematic representation of the analysis of PLP1 gene structure with the ESEfinder, Human Splicing Finder and FAS-ESS programs.

Figure 1

(a) Schematic representation of PLP1 gene structure (7 exons) and the alternative splicing of the PLP1 gene into the major PLP1 transcript and a minor transcript encoding the shorter DM20 isoform, which differs only in terms of the latter half of exon 3 (3B) which is post-transcriptionally spliced out. (b) Only part of the sequence of exon 3A (sequence in black type) and exon 3B (sequence in red type) mutant PLP1 transcript is represented. The mutated nucleotide (c.436C>G) is underlined. According to the ESEfinder 3.0 program, the c.436C>G change created an exonic splicing enhancer (EES, green trapezium), whereas Human Splicing Finder version 2.4.1 and FAS-ESS web server predicted the creation of two exonic splicing silencers (ESS, purple trapezia).