Table 2. Real-time RT-PCR analysis of morpholino-treated Oli-neu cells transfected with mutated (Mut) and wild-type (Wt) PLP1 minigene constructs.

	Mut	2	4	6	8	10	Wt
PLP/(PLP+DM20) ratio	0.002	0.122	0.094	0.079	0.094	0.116	0.212
REC %	0.9	58	44	37	44	55	100

Legend: Columns 2, 4, 6, 8 and 10 indicate samples treated with 10 µM morpholino oligonucleotide in 2, 4, 6, 8 and 10 µM Endoporter reagent, respectively. The PLP/(PLP+DM20) ratio, obtained using the P2 and P23B sets of primers and probes, is reported for each sample. Rec % denotes the proportion of recovered PLP1 transcript expression as compared to the wild-type.