Figure 5. Combination of erlotinib and niclosamide synergistically represses head and neck cancer growth in vivo.

A, Mice bearing Tu212 xenografts were treated with vehicle control, erlotinib (Erlo, 40mg/kg/d), niclosamide (Niclo, 20mg/kg/d) or their combination for 14 days. Each group included 8 mice. Tumor volume was measured once every 2 days. After 14 days, the mice were sacrificed and the tumors were removed and analyzed. Representative tumor pictures were taken. B, Active caspase 3 and Ki 67 were analyzed in tumor tissues at the end of experiments by IHC staining and quantified as described in “Methods”. C, Expression levels of pEGFR, pSTAT3, Bcl2 and Bcl-XL in tumor tissues from various treatment groups were analyzed by Western blot.