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. 2013 Sep 3;8(9):e72797. doi: 10.1371/journal.pone.0072797

Figure 2. Effect of NOS inhibitors on metamorphosis of Herdmania momus.

Figure 2

(A) L-nitroarginine methyl ester (L-NAME), (B) aminoguanidine hemisulfate (AGH), and (C) S-methylisothiourea sulphate (SMIS) were applied at various concentrations to competent larvae (14 hpf). The number of individuals undergoing metamorphosis was counted 4 h after the initiation of exposure (4 hpi). Filtered sea water (FSW) and 40 mM KCl-elevated FSW were used as negative and positive controls, respectively. Data are presented as the mean percentage of larval metamorphosis ± SEM (n = 3 for each treatment, 30 larvae per replicate). Diamonds indicate the actual percentages of larval metamorphosis in each replicate. Letters above error bars indicate statistically significant differences (P<0.05), as determined by one-way analysis of variance and Tukey’s HSD post hoc testing.