Figure 4. Effect of heat-shock on metamorphosis of Herdmania momus.

Larvae were exposed at competency (14 hpf) to one of four temperatures (25, 29, 32 and 35°C) for 2 h. The number of individuals undergoing metamorphosis was counted 24 h after the initiation of heat-shock exposure (24 hpi). FSW and 40 mM KCl-elevated FSW, maintained at the culture temperature of 25°C, were used as negative and positive controls, respectively. Data are presented as the mean percentage of larval metamorphosis ± SEM (n = 3 for each treatment, 30 larvae per replicate). Diamonds indicate the actual percentages of larval metamorphosis in each replicate. Letters above error bars indicate statistically significant differences (P<0.05), as determined by one-way analysis of variance and Tukey’s HSD post hoc testing.